Items with full text/Total items : 67621/67621 (100%)
Visitors : 23089880
Online Users : 299
Please use this identifier to cite or link to this item:
|Title: ||動態干涉儀應用於量測活體微生物之光學常數;Measuring the optical constant of living microorganisms by a Linnik-type dynamic interferometer|
|Authors: ||陳詣欽;Chen, Yi-Qin|
|Keywords: ||動態干涉儀;活體微生物檢測;Linnik-type dynamic interferometer;microorganism measurement|
|Issue Date: ||2019-09-03 14:44:58 (UTC+8)|
微生物公認的細胞質折射率範圍在1.35至1.37之間，本實驗檢測出的微生物細胞質折射率在其範圍內。而為了達到即時檢測折射率的變化，本實驗亦利用演算法取得微生物的厚度，計算出其相位調制級數並輸入程式，即可即時算出整體活體微生物的折射率及厚度，達到即時動態量測效果。;In recent years, due to the number of people who are ill with cancer grow up, many people have studied the difference between cancer cells and normal cells. The differences between cancer cells and normal cells include cell shape, cell size, etc. In avoid to damaging the sample and the position of the nucleus can be clearly distinguished, the position of the nucleus is located by the difference in refractive index between the nucleus and the cytoplasm. At present, most of the instruments for detecting the whole field refractive index are scanning interferometers, and they are susceptible to environmental interference during the detection. It takes a while to obtain the refractive index. This paper will propose a method to solve the environmental interference and quickly obtain the refractive index.
In this experiment, the Linnik-type polarization interferometer combined with the camera produced by 4D Technology Company is used as the experimental framework to effectively reduce the environmental interference, and the biological measurement can be effectively measured even when the optical table is not inflated. In order to test the feasibility of the experimental instrument, the optical film was first to be analyzed. The thickness error was 0.03%, the standard deviation was 0.74 nm, the refractive index error was 0.24%, and the standard deviation was 0.0032.
The recognized cytoplasmic refractive index ranges from 1.35 to 1.37, and the microbial cytoplasmic refractive index detected in this experiment is within its range. In order to achieve the instantaneous detection of the change of refractive index, this experiment uses the algorithm to obtain the thickness of the microorganism, and calculates the phase modulation level. By inputting the phase modulation series into the program, the refractive index and thickness of the whole living microorganism can be calculated instantly.
|Appears in Collections:||[光電科學研究所] 博碩士論文|
Files in This Item:
All items in NCUIR are protected by copyright, with all rights reserved.
::: Copyright National Central University. | 國立中央大學圖書館版權所有 | 收藏本站 | 設為首頁 | 最佳瀏覽畫面: 1024*768 | 建站日期：8-24-2009 :::