| 摘要: | 富含蛋白質的大豆產品，能提供人體營養所需之胺基酸及礦物質，大豆不僅可以降低血清中總膽固醇(Total Cholesterol)、低密度脂蛋白(Low-density lipoproteins) 以及甘油三酸脂(Triglyceride)的濃度，還能提升高密度脂蛋白(High-density lipoproteins)。此外，發酵後的大豆製品亦被證明可有效減輕糖尿病、血壓、心臟疾病以及和癌症相關的問題。經過發酵後的大豆製品，營養價值比未發酵的大豆還來的高，因此，本論文主要利用金花菌發酵黃豆，並探討其組成以及營養價值。
本實驗以市售之黃豆作為原料，將其浸泡12小時後，榨汁製成清豆漿。利用自茶葉分離出的金花菌株(Eurotium cristatum)接種至清豆漿與豆渣發酵三天，並將溫度控制於25℃、30℃、35℃、40℃，搖瓶轉速設定為125rpm，分析其酵素抑制能力、抗氧化活性、總多酚含量以及異黃酮?元轉換率。並發現於30℃、35℃下發酵可得最佳酵素抑制能力與產物濃度及最大菌重濃度。
此外，因藍莓含有豐富的抗氧化物、抗癌物質、多酚類，可以降低心血管和中風等疾病。藍莓中所含之花青素能調節胰島素敏感度，以降低血糖水平，有助於預防及改善第2型糖尿病，還有抑制體內發炎的效果。故將添加藍莓之清豆漿做為實驗對照組，比較其酵素抑制之效果。實驗結果發現於35℃下添加10%豆漿的發酵液有最佳酵素抑制能力及抗氧化力。
;Soy products are rich in protein and have many health benefits. Some researchers have pointed out that soybeans can reduce the serum concentrations of total cholesterol, low-density lipoproteins, and triglycerides in the serum. In addition, fermented soy products have also been proven to be effective in reducing diabetes mellitus, blood pressure, heart disease, and cancer-related problems. Fermented soybean products have higher nutritional value than unfermented soybeans. Therefore, this work is about using Eurotium cristatum to ferment soymilk, and discusses its composition and nutritional value.
In this study, soybeans was raw materials. It was soaked for 12 hours, and then juiced into clear soy milk. Eurotium cristatum was isolated from tea and inoculated to soy milk and soy residue for three days of fermentation, and the temperature was controlled at 25℃、30℃、35℃、40℃ respectively, the shaker’s speed was set at 125rpm, and the samples’ enzyme inhibition ability, antioxidant activity, total polyphenol content and isoflavone bioconversion rate were analyzed. It was found that the optimal enzyme inhibition ability and product concentration and maximum bacterial weight concentration were obtained from fermentation at 30℃ and 35℃.
In addition, blueberries are rich in antioxidants, anti-cancer elements, polyphenols, which can reduce cardiovascular and stroke and other diseases. The anthocyanins contained in blueberries regulate insulin sensitivity to lower blood sugar levels, help prevent and improve type 2 diabetes, and inhibit inflammation in the body. Therefore, the blueberry was added to clear soy milk as an experimental control group, comparing the effect of its enzyme inhibition. The results showed that the fermentation liquid with 10% soy milk at 35℃ had highest enzyme inhibition and antioxidant ability. |
