Flavin mononucleotide-based electricity production by Staphylococcus epidermidis alleviates SARS-CoV-2- Nucleocapsid Phosphoprotein-induced IL-6 expression

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Title:	Flavin mononucleotide-based electricity production by Staphylococcus epidermidis alleviates SARS-CoV-2- Nucleocapsid Phosphoprotein-induced IL-6 expression
Authors:	曾阮梅貞;Trinh, Tang Nguyen Mai
Contributors:	生醫科學與工程學系
Keywords:	SARS-CoV-2-核衣殼磷蛋白;表皮葡萄球菌;黃素單核?酸;電子;SARS-CoV-2-Nuclecapsid phosphoprotein;Staphylococcus epidermidis;flavin mononucleotide;electricity
Date:	2021-10-15
Issue Date:	2021-12-07 11:32:50 (UTC+8)
Publisher:	國立中央大學
Abstract:	嚴重特殊傳染性肺炎是一種引起急性呼吸道窘迫症候群 (ARDS) 的病毒，最初在中國武漢首度被發現。目前嚴重特殊傳染性肺炎這種流行病已成為全球大流行的疾病。根據研究顯示，罹患此病的患者會有呼吸系統及合併多器官功能障礙，此之外也會引起細胞激素釋放症候群(CRS) 的發炎細胞因子增加，發炎細胞因子增加可能在COVID-19 中扮演關鍵作用。根據我們的研究，將濃度 50 μg 的 SARS-CoV-2-核衣殼蛋白 (NPP) 接種到小鼠的鼻腔中可在小鼠支氣管肺泡灌洗液 (BALF) 中存在大量 IL-6，在細胞株實驗中菌株 J774A1 巨噬細胞，接種濃度NPP濃度 25 μg 時菌株 J774A1 巨噬細胞的 IL-6 含量，IL-6 濃度為 402,841 ± 16,713 ρg/mL 和 133,136分別為 ± 2,736 ρg/mL。此外將甘油和黃素單核?酸 (FMN) 在小鼠鼻內接種，從人鼻腔 (S. epidermidis-K) 分離的表皮葡萄球菌預處理 3 天，顯著減少了小鼠體內NPP 誘導的 IL-6 水平。表皮葡萄球菌 K 分泌的電可以通過 FMN 介質降低 SARS-CoV-2-NPP 誘導的 IL-6 表達。;Severe acute respiratory syndrome Coronavirus 2 (SARS-2) is a viral strain that causes acute respiratory distress syndrome (ARDS) and was initially reported in Wuhan, China. This epidemic has been becoming a pandemic worldwide. It has been reported that respiratory syndrome and multi-organ dysfunction in patients, as well as an increase in inflammatory cytokines known as cytokine release syndrome (CRS), may play a crucial role in the detected COVID-19. Our studies have demonstrated that SARS-CoV-2-Nucleocapsid phosphoprotein (NPP) at a concentration of 50 μg was inoculated into the nasal cavity of mice. There was a significant abundance of IL-6 in the bronchoalveolar lavage fluid (BALF) and at a concentration of 25 μg inoculated to increase the IL-6 content of strain J774A1 macrophages, IL-6 concentrations were 402,841 ± 16,713 ρg/mL and 133,136 ± 2,736 ρg/mL, respectively. Furthermore, the administration of glycerol and flavin mononucleotide (FMN) inoculation to mice infected with Staphylococcus epidermidis isolated from human nasal passages (S. epidermidis-K) for 3 days significantly reduced the IL-6 levels induced by NPP. The electricity secreted by S. epidermidis K can reduce SARS-CoV-2-NPP-induced IL-6 expression through FMN mediator.
Appears in Collections:	[Institute of Biomedical Engineering] Electronic Thesis & Dissertation

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