;Three-dimensional bioprinting has evolved popular biomedical engineering in recent years. With the advantages of tissue engineering and additive manufacturing, biological scaffolds can be customized by three-dimensional bioprinting, solve the problem that it is difficult to simulate the natural structure or the manufacture of complex graphics. With the invention of layered manufacturing combined with rotary printing technology, it provides more methods for making small-diameter tubular stents. While improving the success rate of production, it can also maintain the smoothness of the central hole of the tubular stent, making it a suitable tube-shaped stent. The way the stent is made. This study aims to develop the three-dimensional bio-printer system with planar and rotation printing funtions. The bio-printer system can manufacture planar scaffolds and hollow tubular scaffolds on the stable circumstance. We use Ethernet Control Automation Technology (EtherCAT) as the main communication method of software and hardware. We have newly designed several modules, for example: planar and rotation replaceable printing platform module, air curtain enclosed printing environment module, antifreeze defrost module, improved drainage module and infrared temperature sensing module, etc. Finally, we develop the user control interface of C# environment to integrate the software and hardware equipment, and the operator can easily perform with simple training. We observe the generation of air curtain through smoke flow, detect the printing circumstance impact of it, and then we quantify the results and record them. At last, we use the pneumatic extrusion nozzle to pinting the chitosan planar and tubular scaffolds on the working platform and rotating shaft of low temperature, to verify the function of the machine.
