基因表現分析提供許多可能的轉錄因子基因群組，而演化足跡法可用來識別 轉錄因子基因。所以我們開發一個結合尋找具有某種特定功能或構造的片段與演 化足跡法的系統。在輸入一組具有某種特定功能或構造的片段與基因群組，系統 會統計在該片段在人類與老鼠所有基因與基因群上游保守區域的出現情形。系統 可以分辨出有可能是某個轉錄因子調控的基因群組或是沒有關係的基因群組。透 過比較所有基因與基因群組具有某種特定功能或構造的片段分佈情形，本系統可 幫助生物學家識別從基因表現分析得到的群組中，辨識可能是被轉錄因子調控的 基因群組。 The analyses of gene expression provide lots of possible transcription factor regulated gene group. It is proven that phylogenetic footprinting method can help to identify transcription factor binding sites. Our work develops a system that combines the motif searching and phylogenetic footprinting. After a motif and gene group is submitted, the system is able to evaluate statistics of motifs appearance in whole genes and a set of genes upstream conserved region between human and mouse. A transcription factor-target gene group was used to test the proposed system. The results present the system can distinguish transcription factor--target gene group from random gene groups. By comparing motif distribution between whole genes and gene group, the proposed system is able to help biologists identify possible transcription factor regulated genes groups form groups of gene expression.
