光交聯是三維生物列印用來強化生物墨水結構的方法之一，但生物墨水內所添加光啟始劑產生的離子以及紫外光重覆照射，恐怕會降低細胞活性。 本研究發展路徑導引式噴頭，並結合近紅外光飛秒雷射，將光源使用顯微物鏡聚焦成一點，並於焦點前方利用噴頭將生物墨水隨著預定位置擠出，隨後立即使其產生光焦聯。當飛秒雷射照射至光敏材料上能產生雙光子吸收效應，而未被光束照射的區域不會聚合，只有焦點附近的光敏材料會固化，故能更準確地達到局部固化的效果。但因為雷射焦點很小，故需要加裝振鏡，讓雷射來回掃描，以列印出較大尺寸的生物支架。 本研究設計驗證雷射準直與振鏡掃描之雷射焦點範圍，並透過疊圖和橢圓擬合來辨識雷射焦點中心與振鏡掃描的實際範圍。結果證明雷射準直之雷射焦點中心X座標偏差值在三維生物列印機列印平台升降時為55.81 μm；運動平台Z軸升降時為30.03 μm；振鏡掃描時為34.67 μm。透過調整類比電壓輸出由-0.6 V至0.6 V所量測之振鏡掃描實際範圍為1.369 mm，經由以上結果可確保雷射能達到設定之目標。 ;Photocrosslinking is one of the methods used by 3D bioprinting to strengthen the structure of bio-ink, However, repeated irradiation of the ions generated by the photoinitiator added in the bio-ink and UV light may reduce cell activity. In this study, the Path Guiding Nozzle will be developed and combined with the near-infrared femtosecond laser. It can focus the light source into a point using a microscopic objective lens, and push the bio-ink in the predetermined position by the nozzle. Then the material can be crosslinked rapidly. Two-photon polymerization happened when femtosecond laser focuses on the photopolymer material. In contrast to the unexposed place hasn’t been cured. Photopolymer material where be cured only around the focus so can achieve the effect of partial curing more accuracy. Nevertheless, the laser focus is very small. It is needed to install the galvanometer to let the laser scan and print the larger size bio-scaffold. This research designs and confirms the laser collimation and laser focus range of the galvanometer scanning. Using overlay and ellipse fitting to identify the center of the laser focus and the actual range of the galvanometer scan. The result proves that when the printing platform of 3D bioprinter is moved the X coordinate standard deviation of the laser focus center of the laser collimation is 55.81 μm, the Z-axis of the motion platform moved is 30.03 μm, and the galvanometer scanning is 34.67 μm. The actual scanning range of the galvanometer measured by adjusting the analog voltage output from -0.6 V to 0.6 V is 1.369 mm. The above results ensure that the laser can reach the set target.
