摘要： This report describes an enzyme-linked immunosorbent assay (ELISA) for tissue-bound metabolite 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) and the application to residue analysis in cultured fish samples. The residue is monitored as a marker for the drug furaltadone. The assay enables the detection of protein bound AMOZ in the form of a 2-nitrophenyl derivative (2-NP-AMOZ) in sample supernatant or extract after acid hydrolysis and derivatization with o-nitrobenzaldehyde. Polyclonal rabbit antibodies were produced with a new immunogen hapten, 2-NP-HXA-AMOZ. The new ELISA had adequate analytical sensitivity (IC50 value 0.325 μg kg-1; limit of detection 0.1 μg kg-1) to determine a trace of AMOZ residue and had a high selectivity. Recoveries of AMOZ fortified at the levels of 0.1, 0.5 and 1.0 μg kg-1 ranged from 89.8 to 112.5% with coefficients of variation of 12.4-16.2% over the range of AMOZ concentrations studied. The results obtained with the ELISA correlated well with those obtained by commercial test kits for 150 tested samples (r=0.984). The results suggest that the developed ELISA is a highly specific, accurate, and sensitive method suitable for high throughput screening for AMOZ residues. 其他題名： J. Vet. Med. Sci. 出版者： Japan: JAPANESE SOCIETY OF VETERINARY SCIENCE 出版日期： 2012 出處： Journal of Veterinary Medical Science, 2012, Vol.74(11), pp.1439-1446 資源來源： J-STAGE Free 版權： 2012 by the Japanese Society of Veterinary Science 版權： Copyright Japan Science and Technology Agency 2012 識別號： ISSN: 1347-7439 識別號： ISSN: 0916-7250 識別號： EISSN: 1347-7439 識別號： DOI: 10.1292/jvms.12-0165 識別號： PMID: 22785246
