資料載入中.....
|
請使用永久網址來引用或連結此文件:
https://ir.lib.ncu.edu.tw/handle/987654321/101622
|
| 題名: | Green-synthesized silver nanoparticles as a novel control tool against dengue virus (DEN-2) and its primary vector Aedes aegypti |
| 作者: | 樋口亞紺;Sujitha, Vasu;Murugan, Kadarkarai;Paulpandi, Manickam;Panneerselvam, Chellasamy;Suresh, Udaiyan;Roni, Mathath;Nicoletti, Marcello;Higuchi, Akon;Madhiyazhagan, Pari;Subramaniam, Jayapal;Dinesh, Devakumar;Vadivalagan, Chithravel;Chandramohan, Balamurugan;Alarfaj, Abdullah A.;Munusamy, Murugan A.;Barnard, Donald R.;Benelli, Giovanni |
| 貢獻者: | 工學院化學工程與材料工程學系 |
| 關鍵詞: | Aedes - drug effects;Aedes aegypti;Animals;antiviral properties;Biomedical and Life Sciences;Biomedicine;Cercopithecus aethiops;Chemical synthesis;Control;control methods;dengue;Dengue - prevention & control;Dengue - virology;Dengue Virus;energy-dispersive X-ray analysis;Fourier transform infrared spectroscopy;Green Chemistry Technology;Health aspects;Humans;Immunology;Insecticides - pharmacology;instars;Larva - drug effects;larvae;lethal concentration 50;Medical Microbiology;Metal Nanoparticles - chemistry;Methods;Microbiology;Moringa oleifera;Moringa oleifera - chemistry;Mosquitoes;Nanoparticles;nanosilver;Original Paper;Plant Extracts - chemistry;Production processes;public health;pupae;scanning electron microscopy;seed extracts;Seeds - chemistry;serotypes;Silver;Silver - chemistry;Silver - pharmacology;Structure-Activity Relationship;structure-activity relationships;subtropics;vector control;Vero Cells;viral load;X-ray diffraction |
| 日期: | 2015-09-18 |
| 上傳時間: | 2026-04-21 14:39:26 (UTC+8) |
| 出版者: | Springer Verlag;Berlin/Heidelberg: Springer Berlin Heidelberg |
| 摘要: | 摘要： Dengue is an arthropod-borne viral infection mainly vectored through the bite of Aedes mosquitoes. Recently, its transmission has strongly increased in urban and semi-urban areas of tropical and sub-tropical regions worldwide, becoming a major international public health concern. There is no specific treatment for dengue. Its prevention and control solely depends on effective vector control measures. In this study, we proposed the green-synthesis of silver nanoparticles (AgNP) as a novel and effective tool against the dengue serotype DEN-2 and its major vector Aedes aegypti. AgNP were synthesized using the Moringa oleifera seed extract as reducing and stabilizing agent. AgNP were characterized using a variety of biophysical methods including UV–vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), and sorted for size categories. AgNP showed in vitro antiviral activity against DEN-2 infecting vero cells. Viral titer was 7 log₁₀ TCID₅₀/ml in control (AgNP-free), while it dropped to 3.2 log₁₀ TCID₅₀/ml after a single treatment with 20 μl/ml of AgNP. After 6 h, DEN-2 yield was 5.8 log₁₀ PFU/ml in the control, while it was 1.4 log₁₀ PFU/ml post-treatment with AgNP (20 μl/ml). AgNP were highly effective against the dengue vector A. aegypti, with LC₅₀ values ranging from 10.24 ppm (I instar larvae) to 21.17 ppm (pupae). Overall, this research highlighted the concrete potential of green-synthesized AgNP in the fight against dengue and its primary vector A. aegypti. Further research on structure–activity relationships of AgNP against other dengue serotypes is urgently required.
其他題名： Parasitol Res
出版者： Berlin/Heidelberg: Springer Berlin Heidelberg
出版日期： 2015-09-01
出處： Parasitology Research, 2015-09, Vol.114 (9), p.3315-3325
版權： Springer-Verlag Berlin Heidelberg 2015
版權： COPYRIGHT 2015 Springer
識別號： ISSN: 0932-0113
識別號： ISSN: 1432-1955
識別號： EISSN: 1432-1955
識別號： DOI: 10.1007/s00436-015-4556-2
識別號： PMID: 26063530 |
| 顯示於類別: | [化學工程與材料工程學系 ] 期刊論文
|
文件中的檔案:
| 檔案 |
描述 |
大小 | 格式 | 瀏覽次數 |
|---|
| index.html | | 0Kb | HTML | 23 | 檢視/開啟 |
|
在NCUIR中所有的資料項目都受到原著作權保護.
|
