Burkholderia pseudomallei infection induces the expression of apoptosis-related genes and proteins in mouse macrophages

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Title:	Burkholderia pseudomallei infection induces the expression of apoptosis-related genes and proteins in mouse macrophages
Authors:	陳師慶;Hseu, You-Cheng;Sung, Jia-Chuen;Shieh, Bao-Sen;Chen, Ssu Ching
Contributors:	生醫理工學院生命科學系
Keywords:	Animals;Apoptosis;Apoptosis-based polymerase chain reaction array;Blotting, Western;Burkholderia pseudomallei;Burkholderia pseudomallei - pathogenicity;Caspases;Cells, Cultured;Gene Expression Profiling;Host-Pathogen Interactions;Infectious Disease;Macrophages - microbiology;Macrophages - physiology;Medical Education;Mice;Real-Time Polymerase Chain Reaction
Date:	2014-10-01
Issue Date:	2026-04-23 11:11:52 (UTC+8)
Publisher:	Elsevier Taiwan LLC;England: Elsevier B.V
Abstract:	摘要： In this study, we addressed whether the production of apoptosis-related genes and proteins is induced in mouse macrophages infected with Burkholderia pseudomallei cells. Mouse macrophages were infected with B. pseudomallei cells at 0.5 hours, 1 hour, 2 hours, 4 hours, and 6 hours, respectively, followed by real-time polymerase chain reaction (PCR) array analysis. The amount of apoptosis-related proteins (caspase-3, caspase -8, caspase -9, Bax, and Bcl-2) was confirmed by Western blot. After infection, an increase of these proteins was observed. The expression levels of other apoptosis-related genes were also determined by PCR array. Experimental results revealed that the messenger RNA levels of tumor necrosis factor ligand (e.g., tnfsf10 and tnfrs10b) and fas were increased, whereas the expression levels of some antiapoptosis genes such as Birc5, Hells, and Bnip3 were decreased. Our study results demonstrate that the apoptosis-related genes and proteins in mouse macrophages were modulated by B. pseudomallei. 其他題名： J Microbiol Immunol Infect 出版者： England: Elsevier B.V 出版日期： 2014-10-01 出處： Journal of microbiology, immunology and infection, 2014-10, Vol.47 (5), p.394-398 資源來源： Elsevier ScienceDirect Journals Complete 版權： 2013 版權： Copyright © 2013. Published by Elsevier B.V. 識別號： ISSN: 1684-1182 識別號： ISSN: 1995-9133 識別號： EISSN: 1995-9133 識別號： DOI: 10.1016/j.jmii.2013.03.011 識別號： PMID: 23751765
Appears in Collections:	[Department of Life Science] journal & Dissertation

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