Burkholderia pseudomallei infection induces the expression of apoptosis-related genes and proteins in mouse macrophages

NCU Institutional Repository > 生醫理工學院 > 生命科學系 > 期刊論文 > Item 987654321/102497

請使用永久網址來引用或連結此文件: https://ir.lib.ncu.edu.tw/handle/987654321/102497

題名:	Burkholderia pseudomallei infection induces the expression of apoptosis-related genes and proteins in mouse macrophages
作者:	陳師慶;Hseu, You-Cheng;Sung, Jia-Chuen;Shieh, Bao-Sen;Chen, Ssu Ching
貢獻者:	生醫理工學院生命科學系
關鍵詞:	Animals;Apoptosis;Apoptosis-based polymerase chain reaction array;Blotting, Western;Burkholderia pseudomallei;Burkholderia pseudomallei - pathogenicity;Caspases;Cells, Cultured;Gene Expression Profiling;Host-Pathogen Interactions;Infectious Disease;Macrophages - microbiology;Macrophages - physiology;Medical Education;Mice;Real-Time Polymerase Chain Reaction
日期:	2014-10-01
上傳時間:	2026-04-23 11:11:52 (UTC+8)
出版者:	Elsevier Taiwan LLC;England: Elsevier B.V
摘要:	摘要： In this study, we addressed whether the production of apoptosis-related genes and proteins is induced in mouse macrophages infected with Burkholderia pseudomallei cells. Mouse macrophages were infected with B. pseudomallei cells at 0.5 hours, 1 hour, 2 hours, 4 hours, and 6 hours, respectively, followed by real-time polymerase chain reaction (PCR) array analysis. The amount of apoptosis-related proteins (caspase-3, caspase -8, caspase -9, Bax, and Bcl-2) was confirmed by Western blot. After infection, an increase of these proteins was observed. The expression levels of other apoptosis-related genes were also determined by PCR array. Experimental results revealed that the messenger RNA levels of tumor necrosis factor ligand (e.g., tnfsf10 and tnfrs10b) and fas were increased, whereas the expression levels of some antiapoptosis genes such as Birc5, Hells, and Bnip3 were decreased. Our study results demonstrate that the apoptosis-related genes and proteins in mouse macrophages were modulated by B. pseudomallei. 其他題名： J Microbiol Immunol Infect 出版者： England: Elsevier B.V 出版日期： 2014-10-01 出處： Journal of microbiology, immunology and infection, 2014-10, Vol.47 (5), p.394-398 資源來源： Elsevier ScienceDirect Journals Complete 版權： 2013 版權： Copyright © 2013. Published by Elsevier B.V. 識別號： ISSN: 1684-1182 識別號： ISSN: 1995-9133 識別號： EISSN: 1995-9133 識別號： DOI: 10.1016/j.jmii.2013.03.011 識別號： PMID: 23751765
顯示於類別:	[生命科學系] 期刊論文

文件中的檔案:

檔案	描述	大小	格式	瀏覽次數
index.html		0Kb	HTML	19	檢視/開啟

在NCUIR中所有的資料項目都受到原著作權保護.

社群 sharing

資料載入中.....