| 摘要: | 摘要： The effects and possible underlying mechanism of curcumin combined with radiation in human hepatocellular carcinoma (HCC) cells in vitro were evaluated. The effects of curcumin, radiation, and combination of both on cell viability, apoptosis, NF-κB activation, and expressions of NF-κB downstream effector proteins were investigated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), NF-κB reporter gene, mitochondrial membrane potential (MMP), electrophoretic mobility shift (EMSA), and Western blot assays in Huh7-NF-κB-luc2, Hep3B, and HepG2 cells. Effect of I kappa B alpha mutant (IκBαM) vector, a specific inhibitor of NF-κB activation, on radiation-induced loss of MMP was also evaluated. Results show that curcumin not only significantly enhances radiation-induced cytotoxicity and depletion of MMP but inhibits radiation-induced NF-κB activity and expressions of NF-κB downstream proteins in HCC cells. IκBαM vector also shows similar effects. In conclusion, we suggest that curcumin augments anticancer effects of radiation via the suppression of NF-κB activation.
其他題名： Biomed Res Int
出版者： Cairo, Egypt: Hindawi Publishing Corporation
出版日期： 2015-01-01
出處： BioMed research international, 2015-01, Vol.2015 (2015), p.1-7
資源來源： Academic Search Premier (Ebsco)
版權： Copyright © 2015 Fei-Ting Hsu et al.
版權： COPYRIGHT 2015 John Wiley & Sons, Inc.
版權： Copyright © 2015 Fei-Ting Hsu et al. 2015
識別號： ISSN: 2314-6133
識別號： ISSN: 2314-6141
識別號： EISSN: 2314-6141
識別號： DOI: 10.1155/2015/363671
識別號： PMID: 26539482 |
