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| 題名: | Functional divergence of the miRNA transcriptome at the onset of drosophila metamorphosis |
| 作者: | 葉淑丹;Yeh, Shu-Dan;von Grotthuss, Marcin;Gandasetiawan, Kania A.;Jayasekera, Suvini;Xia, Xiao-Qin;Chan, Carolus;Jayaswal, Vivek;Ranz, José M. |
| 貢獻者: | 生醫理工學院生命科學系 |
| 關鍵詞: | Animals;Biological evolution;Conserved Sequence;Developmental stages;Drosophila;Drosophila melanogaster;Drosophila melanogaster - classification;Drosophila melanogaster - genetics;Drosophila melanogaster - growth & development;Ecdysone;Evolution, Molecular;Female;Fruit flies;Gene expression;Gene Expression Profiling;Gene Expression Regulation, Developmental;Genetic Variation;Insects;Male;Metamorphosis;Metamorphosis, Biological;MicroRNAs;MicroRNAs - genetics;miRNA;Molecular biology;Molecular Sequence Data;Molecules;Ontogeny;Phylogeny;Polymorphism;Sex Characteristics;Transcriptomes;Variation |
| 日期: | 2014-10-01 |
| 上傳時間: | 2026-04-23 11:12:47 (UTC+8) |
| 出版者: | Oxford University Press;United States: Oxford University Press |
| 摘要: | 摘要： MicroRNAs (miRNAs) are endogenous RNA molecules that regulate gene expression posttranscriptionally. To date, the emergence of miRNAs and their patterns of sequence evolution have been analyzed in great detail. However, the extent to which miRNA expression levels have evolved over time, the role different evolutionary forces play in shaping these changes, and whether this variation in miRNA expression can reveal the interplay between miRNAs and mRNAs remain poorly understood. This is especially true for miRNA expressed during key developmental transitions. Here, we assayed miRNA expression levels immediately before (≥18BPF [18 h before puparium formation]) and after (PF) the increase in the hormone ecdysone responsible for triggering metamorphosis. We did so in four strains of Drosophila melanogaster and two closely related species. In contrast to their sequence conservation, approximately 25% of miRNAs analyzed showed significant within-species variation in male expression levels at ≥18BPF and/or PF. Additionally, approximately 33% showed modifications in their pattern of expression bias between developmental timepoints. A separate analysis of the ≥18BPF and PF stages revealed that changes in miRNA abundance accumulate linearly over evolutionary time at PF but not at ≥18BPF. Importantly, ≥18BPF-enriched miRNAs showed the greatest variation in expression levels both within and between species, so are the less likely to evolve under stabilizing selection. Functional attributes, such as expression ubiquity, appeared more tightly associated with lower levels of miRNA expression polymorphism at PF than at ≥18BPF. Furthermore, ≥18BPF- and PF-enriched miRNAs showed opposite patterns of covariation in expression with mRNAs, which denoted the type of regulatory relationship between miRNAs and mRNAs. Collectively, our results show contrasting patterns of functional divergence associated with miRNA expression levels during Drosophila ontogeny.
其他題名： Mol Biol Evol
出版者： United States: Oxford University Press
出版日期： 2014-10-01
出處： Molecular biology and evolution, 2014-10, Vol.31 (10), p.2557-2572
版權： The Author 2014. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. All rights reserved. For permissions, please e-mail: journals.permissionsoup.com 2014
版權： The Author 2014. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. All rights reserved. For permissions, please e-mail: journals.permissionsoup.com.
版權： Copyright Oxford Publishing Limited(England) Oct 2014
識別號： ISSN: 0737-4038
識別號： ISSN: 1537-1719
識別號： EISSN: 1537-1719
識別號： DOI: 10.1093/molbev/msu195
識別號： PMID: 24951729 |
| 顯示於類別: | [生命科學系] 期刊論文
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