The Optical Society;United States: Optical Society of America
摘要:
摘要： A two-photon hyperspectral microscope with non-de-scanned geometry and parallel recording scheme was constructed which had a low dwell time, high spectral resolution, and high spatial resolution. The dwell time was effectively decreased to reduce photodamage through parallel recording, while the non-de-scanned geometry led to a considerable reduction in the signal loss and spectrum distortion. Two experiments were performed to show that this system can solve crosstalk issues and spectrally resolve the intrinsic fluorophores in optically-thick tissues without staining and sectioning. 其他題名： Biomed Opt Express 出版者： United States: Optical Society of America 出版日期： 2014-02-01 出處： Biomedical optics express, 2014-02, Vol.5 (2), p.338-347 資源來源： Optica Publishing Group Journals 版權： 2014 Optical Society of America 2014 Optical Society of America 識別號： ISSN: 2156-7085 識別號： EISSN: 2156-7085 識別號： DOI: 10.1364/BOE.5.000338 識別號： PMID: 24575331
