The Optical Society;United States: Optical Society of America
摘要:
摘要： In this study, the light diffraction of temporal focusing multiphoton excitation microscopy (TFMPEM) and the excitation patterning of nonlinear structured-illumination microscopy (NSIM) can be simultaneously and accurately implemented via a single high-resolution digital micromirror device. The lateral and axial spatial resolutions of the TFMPEM are remarkably improved through the second-order NSIM and projected structured light, respectively. The experimental results demonstrate that the lateral and axial resolutions are enhanced from 397 nm to 168 nm (2.4-fold) and from 2.33 μm to 1.22 μm (1.9-fold), respectively, in full width at the half maximum. Furthermore, a three-dimensionally rendered image of a cytoskeleton cell featuring ~25 nm microtubules is improved, with other microtubules at a distance near the lateral resolution of 168 nm also able to be distinguished. 其他題名： Biomed Opt Express 出版者： United States: Optical Society of America 出版日期： 2014-08-01 出處： Biomedical optics express, 2014-08, Vol.5 (8), p.2526-2536 資源來源： Optica Publishing Group Journals 版權： 2014 Optical Society of America 2014 Optical Society of America 識別號： ISSN: 2156-7085 識別號： EISSN: 2156-7085 識別號： DOI: 10.1364/BOE.5.002526 識別號： PMID: 25136483
