| 摘要: | 摘要： ABSTRACT<jats:label />A tissue engineering chondrocytes/scaffold construct provides a promise to cartilage regeneration. The architecture of a scaffold such as interconnections, porosities, and pore sizes influences the fates of seeding cells including gene expression, survival, migration, proliferation, and differentiation thus may determine the success of this approach. Scaffolds of highly ordered and uniform structures are desirable to control cellular behaviors. In this study, a newly designed microfluidic device based on flow‐focusing geometry was developed to fabricate gelatin scaffolds of ordered pores. In comparison with random foam scaffolds made by the conventional freeze‐dried method, honeycomb‐like scaffolds exhibit higher swelling ratio, porosity, and comparable compressive strength. In addition, chondrocytes grown in the honeycomb‐like scaffolds had good cell viability, survival rate, glycosaminoglycans production, and a better proliferation than ones in freeze‐dried scaffolds. Real‐time PCR analysis showed that the mRNA expressions of aggrecan and collagen type II were up‐regulated when chondrocytes cultured in honeycomb‐like scaffolds rather than cells cultured as monolayer fashion. Oppositely, chondrocytes expressed collagen type II as monolayer culture when seeded in freeze‐dried scaffolds. Histologic examinations revealed that cells produced proteoglycan and distributed uniformly in honeycomb‐like scaffolds. Immunostaining showed protein expression of S‐100 and collagen type II but negative for collagen type I and X, which represents the chondrocytes maintained normal phenotype. In conclusion, a highly ordered and honeycomb‐like scaffold shows superior performance in cartilage tissue engineering. Biotechnol. Bioeng. 2014;111: 2338–2348. © 2014 Wiley Periodicals, Inc.
其他題名： Biotechnol. Bioeng
出版者： United States: Blackwell Publishing Ltd
出版日期： 2014-11
出處： Biotechnology and Bioengineering, 2014-11, Vol.111 (11), p.2338-2348
資源來源： Wiley Online Library
版權： 2014 Wiley Periodicals, Inc.
版權： Copyright John Wiley and Sons, Limited Nov 2014
識別號： ISSN: 0006-3592
識別號： ISSN: 1097-0290
識別號： EISSN: 1097-0290
識別號： DOI: 10.1002/bit.25295
識別號： PMID: 24895237
識別號： CODEN: BIBIAU |
