摘要： Mesoporous silica nanoparticles (MSNs) have received intensive recent interest as multi-functional nanocarriers for biological cells and tissues. Their cell uptake and intracellular distribution depends on the surface charge they carry. We develop a new approach for monitoring their uptake and intracellular localization by measuring the local pH value of the MSNs. We synthesized a ratiometric pH-sensor loaded with mesoporous silica nanoparticles that can map the location of the MSN through the site pH in HeLa cells. Positively charged MSN-TA was found to locate in higher pH regions which are identified as cytosol mostly. On the other hand, negatively charged MSN-PPs were trapped in acidic endosomes. Evidence is given for direct penetration of membranes through a charge-mediated membrane–nanoparticle interaction mechanism. 出版日期： 2012-02-07 出處： RSC advances, 2012-02, Vol.2 (3 p.968-973), p.968-973 資源來源： Royal Society of Chemistry 識別號： ISSN: 2046-2069 識別號： EISSN: 2046-2069 識別號： DOI: 10.1039/c1ra00586c
