摘要： To investigate the effect of fluorescent probe on the properties of membranes, we studied model membranes composed of 1,2- dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1-palmitoyl 2-oleoyl-sn-glycero-3-phosphocholine (POPC) in the presence and absence of fluorescent probe. The morphology of giant unilamellar vesicles (GUVs) has been observed as a function of temperature and composition by fluorescence microscopy using NBD-DOPE or C 6 -NBD-PC as the probe. The phase behavior of model membranes containing no fluorescent probe was investigated by 2 H-NMR spectroscopy. We found that the bright phase observed on GUVs was the fluid phase enriched in POPC and the dark phase was the gel phase enriched in DPPC. NBD-DOPE and C 6 -NBD-PC preferentially participated in the fluid-phase domains when GUVs were in the gel + fluid phase coexistence. Inclusion of both fluorescent probes (1 mol%) lowered the transition temperature of POPC/DPPC membranes. In addition, C 6 -NBD-PC exhibited a stronger effect than NBD-DOPE, which was considered to be associated with the structures of fluorescent molecules. 其他題名： Mol Membr Biol 出版者： England: Taylor & Francis 出版日期： 2016-02-17 出處： Molecular membrane biology, 2016-02, Vol.33 (1-2), p.23-28 資源來源： EBSCOhost Academic Search Premier 版權： 2016 Informa UK Limited, trading as Taylor & Francis Group 2016 識別號： ISSN: 0968-7688 識別號： ISSN: 1464-5203 識別號： EISSN: 1464-5203 識別號： DOI: 10.1080/09687688.2016.1185175 識別號： PMID: 27452979
