表面電漿共振儀之動態相位偵測技術; 與微量生物分子檢測應用 Dynamic Phase-Acquisition Technique and Low-Abundance Biomolecule Detection of a Surface-Plasmon-Resonance System

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题名:	表面電漿共振儀之動態相位偵測技術;與微量生物分子檢測應用 Dynamic Phase-Acquisition Technique and Low-Abundance Biomolecule Detection of a Surface-Plasmon-Resonance System
作者:	謝佾儒;Yi-ru Hsieh
贡献者:	光電科學研究所
关键词:	表面電漿共振系統;胎兒纖維結合素;Fetal fibronectin;Surface-Plasmon-Resonance system
日期:	2010-10-19
上传时间:	2010-12-08 14:22:51 (UTC+8)
出版者:	國立中央大學
摘要:	本論文以研究團隊所研製之相位式表面電漿共振儀為基礎，開發動態相位偵測技術以提升系統折射率解析度後，並著手進行生物分子的實驗量測。目前，本系統之相位偵測穩定度在2000秒內可達0.5859度，所對應的系統折射率解析度為 RIU(Refraction Index Unit)。以本系統進行胎兒纖維結合素之抗體抗原專一性鍵結相位檢測，當抗原濃度為50 、2.5 、1.25 時，其相位變化分別為3.0154度、1.0613度、9.729度，並由四層結構系統進行數值擬合，擬合之結果得濃度為50 時，鍵結等效折射率為1.33318，等效厚度為10.81 nm；濃度為2.5 時，擬合之等效折射率為1.33297，等效厚度為10.43 nm；濃度為1.25 時，擬合之等效折射率為1.33227，等效厚度為9.84 nm。可知相位訊號、等效折射率及等效厚度均隨抗原濃度變化，有機會經由系統校正程序獲得關係曲線後，應用此系統進行抗原濃度檢測。 In this thesis, a dynamic phase-detection technique was developed to improve the refractive-index resolution of a surface-plasmon-resonance system previously built in the research group. After examining the system stability, biological molecule measurements were performed. The phase-detection stability was 0.5859 degree in 2000-second period, and the corresponding refractive-index resolution was 1.3×10-5 RIU (Refraction Index Unit). This system was applied to antibody-antigen binding experiments of fetal fibronectin. With antigen concentrations of 50 ng/ml, 2.5 ng/ml, and 1.25 ng/ml, the phase changes were 3.0154, 1.0613 and 9.729 degrees, respectively. Considering a four-layer Kretschmann configuration, the equivalent thickness and the equivalent refractive index of the antibody-antigen binding layer were found by numerical fitting. For the concentration of 50 ng/ml, the equivalent refractive index was 1.33318 RIU and the equivalent thickness was 10.81 nm. For the concentration of 2.5 ng/ml, the equivalent refractive index was 1.33297 RIU and the equivalent thickness was 10.43 nm. For the concentration of 1.25 ng/ml, the equivalent refractive index was 1.33227 RIU and the equivalent thickness was 9.84 nm. As the phase signal, the equivalent refractive index and the equivalent thickness vary with the antigen concentrations, it is possible to obtain their relation curves through calibrations and then apply the system to determinations of the antigen concentration.
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