利用網印碳電極以交流阻抗法檢測糖化血紅素; Glycated Hemoglobin Detection with Screen-printed Carbon Electrode by AC Impedance Analysis

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Title:	利用網印碳電極以交流阻抗法檢測糖化血紅素;Glycated Hemoglobin Detection with Screen-printed Carbon Electrode by AC Impedance Analysis
Authors:	魏永年;Yung-nian Wei
Contributors:	電機工程研究所
Keywords:	交流阻抗;網印電極;血紅素;糖化血紅素;screen-printing;electrode;hemoglobin;glycated hemoglobin;AC impedance analysis
Date:	2011-08-24
Issue Date:	2012-01-05 14:58:29 (UTC+8)
Abstract:	本研究利用拋棄式碳電極作為量測工具、交流阻抗法作為檢測分析方法，對血紅素(Hb)與糖化血紅素(HbA1c)進行檢測。拋棄式碳電極以網印方式製成，製作出的電極選用具有較高的相對阻抗差進行實驗量測。由於檢測目標物血紅素與糖化血紅素電特性的不同，在溶液中會產生不同數量的帶電粒子。利用交流阻抗法量測將會由等效電路中元件的改變得到穩定顯著的阻抗差異。藉由此原理可以得知Hb、 HbA1c最大檢測靈敏度範圍與靈敏度。樣本溶血物(hemolystates)在HbA1c不同含量下時，同樣利用交流阻抗法也可得到HbA1c含量不同所相應的阻抗差關係。並藉由交流阻抗法量測原理建立自製阻抗量測平台電路，利用微控制器負責資料傳輸與控制，運用MATLAB進行資料收集與處理。自製電路針對HbA1c量測結果與現行阻抗儀有相近的趨勢，擁有可以彌補現行儀器不易攜帶、昂貴…等問題，搭配拋棄式碳電極可以達成簡易、快速、成本低、攜帶方便的糖化血紅素檢測系統。 This　research　focused　on　hemoglobin　(Hb)　and　glycated　hemoglobin　(HbA1c)　detection　using　disposable　carbon　electrodes　and　AC　impedance　analysis.　The　disposable　carbon　electrodes　were　fabricated　by　screen-printing　technology.　Among　the　three　fabricated　electrode　configurations,　the　one　that　led　to　the　largest　relative　impedance　difference　was　used　in　the　measurement.　The　natures　of　Hb　and　HbA1c　are　different.　They　have　diffreent　amount　of　charged　particles　in　the　solution.　By　AC　impedance　analysis,　we　can　obtain　stable　and　significant　change　in　the　solution　resistance　in　the　equivalent　circuit　model.　By　this　way　we　knew　the　concentration　range　of　Hb　and　HbA1c　corresponding　to　the　maximum　detection　sensitivity.　A　self-made　circuit　was　used　in　the　impedance　measutement.　A　microcontroller　was　responsible　for　measurement　control　and　data　transmission.　Measurement　data　were　saved　and　processed　with　MATLAB.　The　HbA1c　measurement　results　were　similar　with　the　self-made　citcuit　and　with　the　commercail　impedance　analyser,　which　is　expensive　and　not　portable.　The　self-made　citcuit　and　disposable　carbon　electrodes　have　made　the　measurement　easy,　fast,　inexpensive,　and　portable.
Appears in Collections:	[Graduate Institute of Electrical Engineering] Electronic Thesis & Dissertation

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