| 摘要: | 蒙古黃耆 (學名：Astragalus mongholicus，AM) ，在過去數篇文章中指出將它用於治療癌症有一定的功效[1-4]，但其中的分子機轉的部分仍然尚未明確。本研究目的是欲找出黃耆加藥後影響大腸癌細胞以及組織的分子機轉，用系統生物的分析技術去研究黃是是如何影響或治療大腸癌。本研究先從基因晶片分析開始，使用GeneSpring軟件GX 7.3版（安捷倫，CA，USA）先行選出差異基因，並使用miRTarBase生物資料庫合併分析，最後再結合曾愛倫博士的論文內容合併篩選出候選的基因---的miR-29a[5]，一個非編碼RNA，以進行後續的實驗。本研究使用即時聚合酶鏈鎖反應實驗先去證明在大腸癌HCT116細胞株以及使用本研究室過去研究生曾愛倫留下來的24隻小鼠組織樣本，這些組織樣本是先將牠們皮下注入HCT116細胞並長出腫瘤，再接受黃耆加藥治療，最後在將腫瘤組織處理研究。組織中的miR-29a的表現量有因黃耆造成出現抑制的影響。之後，由於本研究很榮幸能跟陳文逸老師實驗室有合作，因此本研究得以獲得陳老師的專利nDNA修飾的探針使用的權利，以用於進行原位雜交實驗。我們可以確認的miR-29a在HCT116細胞株中表現量有因黃耆加藥後而產生差異。之後本實驗使用先前本研究室留下的小鼠組織陣列晶片去進行組織學原位雜交實驗，進一步去證明黃耆加藥後，在組織學上亦可以看出黃耆加藥之後造成的miR-29a表現量的抑制。或許在未來的miR-29a的標靶藥物會結合黃耆開發出新的醫學以及商業用途用來治療大腸癌。;Astragalus Mongholicus (AM), has pointed out in the past several articles that it has effect on the treatment of colorectal cancer, but the molecular mechanism of the part is still unclear. The purpose of this study is to find out the molecular mechanism of the influence of jaundice on colorectal cancer cells and tissues, and to study how Astragalus mongholicus affects or treats colorectal cancer from the perspective of molecular biology. This study begins with the analysis of the gene chip, uses the GeneSpring software GX version 7.3 (Agilent, CA, USA) to select the differential genes first, and uses the miRTarBase biological database to combine the analysis, and finally combines the previous research results of the laboratory to select the candidate genes. --- miR-29a, a non-coding RNA for subsequent experiments. In this study, the real-time polymerase chain reaction assay was used to prove that the HCT116 cell line of colorectal cancer and the 24 Rats left by the graduate students Zeng Heather in this laboratory were injected subcutaneously into HCT116 cells and grew tumors, and then received jaundice. Drug treatment, and finally in the treatment of tumor tissue. The amount of miR-29a in the tissue is affected by the inhibition caused by jaundice. Later, because this study has cooperated with Chen Wen yih laboratory, this study obtained the right to use Chen′s patented nDNA modified probe for in situ hybridization experiments. We can confirm that the expression of miR-29a in HCT116 cell line is different due to the addition of jaundice. After this experiment, the mouse tissue array wafer left by the previous laboratory was used for histological in situ hybridization experiment, and further proved that after the administration of jaundice, the miR caused by the administration of jaundice can also be seen in histology. -29a inhibition of the amount of expression. Perhaps in the future, miR-29a′s target drugs will be combined with Astragalus to develop new medical and commercial uses for the treatment of colorectal cancer. |
