| 摘要: | 表面增強拉曼散射（Surface-enhanced Raman scattering, SERS）技術作為通過在單分子水平上收集分子光譜信號來識別分子物種一種強而有力的工具，在環境科學、醫學診斷、食品安全以及生物分析等多個領域均取得了重要的研究進展。SERS 技術不僅具有無損檢測樣本、高靈敏度和高選擇性的優勢，同時在分子結構表徵、濃度定量和多物種分析方面展現出廣闊的前瞻性。隨著相關研究逐步深入，越來越多高性能或多功能的 SERS 基底材料被開發出來，這些材料包含但不限於金屬納米結構、金屬-半導體複合材料以及其他新型複合基底，這不僅大幅提升了 SERS 感測的靈敏度和穩定性，也有助於擴展其應用範圍，將其推向更具挑戰性的研究領域。特別是在生物分析領域，SERS 技術已逐漸成為分子檢測和生物標誌物識別的重要手段，能夠在超低濃度條件下實現對蛋白質、核酸、生物大分子等複雜體系的痕量分析，對疾病的早期診斷、癌症標誌物的檢測以及藥物代謝過程的監測等提供了高效而精準的應用潛力 [1]。
在本篇研究中，採用銀奈米粒子並利用多元醇法(Polyol Method)合成的雪花狀銀奈米顆粒(Snowflake-like Silver Nanoparticles, AgNS)與氧化石墨烯(Graphene Oxide, GO)共修飾於濾紙上作為表面增強拉曼(Surface Enhanced Raman Scattering, SERS)基材，以最大限度地減少奈米銀的團聚之外，其複合材料也表現出其他性能，例如對微量重金屬離子的良好光學敏感性、吸附親和力以及去除有機化合物的催化活性。實驗結果顯示，本方法相比僅有銀奈米粒子的基材，其增強因子(Enhancement Factor, EF)提升約1.2×10⁴倍，展現更強的訊號增強效果。本研究證實了此低成本、高靈敏度的複合基材具有應用於小樣本體積高效分析的潛力，為表面增強拉曼技術的改進與發展提供了重要的實驗基礎。
;Surface-enhanced Raman scattering (SERS) technology is a powerful tool for identifying molecular species by collecting spectral signals from individual molecules. It has made significant advancements in various fields, such as environmental science, medical diagnostics, food safety, and bioanalysis. SERS offers benefits including non-destructive detection, high sensitivity, and high selectivity, and it shows great potential for applications in molecular structure characterization, concentration quantification, and multi-species analysis. As research in this area progresses, a growing number of high-performance and multifunctional SERS substrate materials have been developed. These materials include metallic nanostructures, metal-semiconductor composites, and various other novel composite substrates. These advancements have greatly improved the sensitivity and stability of SERS sensing, while also expanding its range of applications, particularly in more challenging research areas. In bioanalysis, SERS technology has become an essential method for molecular detection and biomarker identification. It allows for trace analysis of complex systems, including proteins, nucleic acids, and biomacromolecules, even at ultra-low concentrations. This capability offers promising applications for early disease diagnosis, cancer biomarker detection, and monitoring drug metabolism processes [1] .
In this study, silver nanoparticles synthesized using the polyol method were fabricated into Snowflake-like Silver Nanoparticles (AgNS) and combined with Graphene Oxide (GO) on filter paper as a substrate for surface-enhanced Raman scattering (SERS). This approach minimizes the agglomeration of silver nanoparticles and endows the composite material with additional properties, such as high optical sensitivity to trace heavy metal ions, adsorption affinity, and catalytic activity to remove organic compounds. Raman analysis results show that the newly developed GO and AgNS composite substrate exhibits a stronger signal enhancement effect than substrates with silver nanoparticles alone, achieving an Enhancement Factor (EF) of 1.2 × 10⁴. By employing the SERS technique combined with a low-cost, highly sensitive substrate, small sample volumes were analyzed efficiently, confirming the applicability of snowflake-like silver nanoparticles and graphene oxide in surface-enhanced Raman applications. |
